Sonntag, 24. Januar 2016

Separation of Chlorophyll Pigments by Paper

In my biology lesson we did a chromatography of chloroplast extract:

At first we added a spatula of anhydrous sodium sulphate into an Eppendorf tube.
Then we placed small pieces of spinach leaves into the tube and 5 drops of solvent A (2 parts ethyl ethanoate and 3 parts propanone) and stired al of it a metal probe:

Now we placed a dot of the solvent on a TLC plate with 5 mm space from the bottom edge:

After this we used a dropper pipette to place solvent B(5:3:2 mixture of cyclohexane, ethyl ethanoane and propanone) into a new vial (5mm hight of solution).
Finally we put the TLC plate into the vial and waited for a few minutes, so that the result was as shown here:




1 Kommentar:

  1. I really like how you have not only provided a method as to how to complete this practical but you have also given clear step by step pictures. Easy to follow, I really enjoy your blog.

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